High Efficiency Biological Air Sampler


Excellence in air sampling

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High Efficiency Biological Air Sampler


The High Efficiency Biological Air Sampler (HEBAS) detects potential contamination from viable particles.

The geometry of the HEBAS head is designed to precisely direct sample air onto the culture media surface to assure high collection efficiency (validated according to ISO 14698/1 Annex B).
An added benefit is the reduction of the sample air velocity which minimizes the severe stress and trauma of each micro-organism upon impact.

The HEBAS’ proprietary design will clearly differentiate sample and post-collection contaminants. Any contaminants added outside the controlled environment will be quickly and easily identified. This feature significantly reduces the risk of false positives.

HEBAS is made with fully autoclavable AISI 316L Stainless Steel. The locking system utilizing magnets provides an easy and safe opening and closing operations, with the assurance of perfect sanitization due to the incorporation of the magnets inside the stainless steel.

The HEBAS uses the industry standard 90 mm petri dish for continuous and repeatable sample collection.

When equipped with the Internal Vacuum Source (IVS) unit, the HEBAS provides a self-contained system for the continuous sampling of viable particles within critical environments. The HEBAS IVS is ideal for monitoring in those applications where containment has to be assured (isolators, RABS).

The HEBAS IVS contains an internal power supply and a blower, eliminating the risk associated with using a vacuum source located outside the containment area.

Rigel’s HEBAS IVS provides an added measure of safety by incorporating features resulting in the exhaust air passing through a filtration subsystem certified to ISO 3 compliance.

High Efficiency Biological Air Sampler


Technical specifications

Field of use
Inertial impact device on Petri dishes that allows the capture of airborne particles, for the determination of microbiological contamination associated with particle material.
ISO certification
The impactor meets the requirements of ISO 14698 and has been certified for efficiency by the Health Protection Agency (Portdown – UK) according to Annex B of ISO 14698-1 (report no. 80-10A).
Physical efficiency
D50 (dynamic cut-off point, physical efficiency 50%): 0,8 μm D100 (dynamic cut-off point, physical efficiency 100%): 1,1 μm
Microbiological efficiency
98.9% compared with Casella reference sampler
Sample flow
1.0 CFM (28.3 litres/min) ± 5%
stainless steel AISI 316L
Removable upper part bound to the fixed lower part by means of magnets incorporated in the structure. Separation/re-coupling with simple rotation.
Structure and materials resistant to autoclave treatment.
Diameter 110 mm, height 32 mm
0,9 kg
Standard Petri dishes 90 mm

IVS (Internal Vacuum Source) Unit

Field of use
Unit including internal vacuum pump, suitable for sampling aerosols on HEBAS-type impactors
Rated flow rate
28.3 L /min (1 CFM)
Flow Control
Automatic operational Flow Control (pump feed electronic control ±10%)
Vacuum pump
Brushless type
Flow measurement
Mass flow meter Analog Output 0 ÷ 5 Vdc Range 0 ÷ 32.6 L/min
Start command
5÷32 Vdc (required to activate Sampling cycle)
Input/output signal socket
DIN Male 4 poles M16 IP 40
LED indicators
“POWER” : ON (active sampling cycle) “FLOW STATUS”: ON (flow range 10% of nominal value)
Power supply
100÷240 V 50-60 Hz 30VA
Electrical protection
2 fuses 5×20 mm 250V F 2A
Environmental conditions in the operating phase
Temperature from 0 °C to + 37 °C Relative humidity 10 ÷ 90%
Ambient storage conditions
Temperature from – 20 °C to + 55 °C Relative humidity 10 ÷ 90% non-condensing
Instrument casing
Stainless steel
Impactor line connection
Tri-clamp ¾ “
Dimensions (L x W x H)
160x250x215 mm
2.9 kg (without HEBAS impactor)
First calibration at the manufacturer’s laboratory Calibration frequency: recommended every 12 months

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